Green analytical technologies for the determination of a bioactive compound diosmin\n(DIOM) in the real samples of pharmaceutical formulations and biological fluids are scarce in literature.\nTherefore, the present investigation was carried out to develop a novel, rapid, simple, and economical\ngreen â??reversed phase high-performance thin-layer chromatography (RP-HPTLC)â? method for the\ndetermination of DIOM in commercial tablets and in-house developed spray-dried microparticles\n(MPs). The quantification of DIOM was conducted via â??RP-18 silica gel 60 F254S HPTLC platesâ?.\nThe binary combination of green solvents, i.e., ethanol:water (5.5:4.5 v/v) was proposed as a green\nmobile phase. The analysis of DIOM was conducted in absorbance/reflectance mode of densitometry\nat Lmax = 348 nm. The densitograms of DIOM from the commercial tablets and in-house developed\nspray-dried MPs were verified by recording their single band at Rf = 0.80 0.02 compared to\nstandard DIOM. Green RP-HPTLC method was observed as linear in the range of 100â??700 ng/band\nwith R2 = 0.9995. The proposed method was found as â??accurate, precise, robust, and sensitiveâ?\nfor the determination of DIOM in the real samples of commercial tablets and in-house developed\nspray-dried MPs. The % content of DIOM in the real samples of commercial tablets and in-house\ndeveloped spray-dried MPs was obtained as 99.06 and 101.30%, respectively. The recorded results\nof this research suggested that the green RP-HPTLC method can be eectively used for the routine\nanalysis of DIOM in pharmaceutical products.
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